Blastocyst Transfer

Blastocyst Transfer

In the past most embryos produced with IVF were transferred on day three of development, known as cleavage stage. When an embryo reaches five days of development it is called a blastocyst. Currently, with advances in understanding the needs of developing embryos, the ability to produce blastocysts in the laboratory has increased. This extended culture time allows nature to help select those embryos with the highest capacity to produce a pregnancy. Culturing and transferring blastocysts on day five of development allows the transfer of fewer embryos while still maintaining a high pregnancy rate. Normally only two blastocyst stage embryos are transferred, thus reducing the risk of multiple pregnancies not higher than twins.

Who Need It?

Women at least with few good quality embryos on day 3 after egg collection will have chance of blastocyst transfer

Couples who have undergone repeated failed IVF are also candidates for the blastocyst transfer

For young women having good prognosis. However it is suitable for women of all age groups.

Especially women over 40 years can also benefit and pregnancy rates are high if their embryos are suitable for this procedure.

The Process

The slow and poor embryo stops and embryos of good quality go on to day 5 blastocyst stage. This is the nature s selection of best embryos.

Later best 2 embryos are transferred in to the uterus to achieve pregnancy. 70 – 80 % of pregnancy rate at Fertility Centre is due to the fact that we are specialized in blastocyst transfer with new specialized embryo scope.

We can visualize the blossoming of the 2 cell embryo to a healthy blastocyst which ensures good pregnancy rates.

LAH or Laser Assisted Hatching

LAH is laser assisted hatching is the art of hatching the embryo. A small hole is made in the shell of the embryo (zonapellucida) using special laser and micromanipulation techniques. This facilitates embryo hatching and implantation.

We also use the laser for sperm immobilization. The sperm is immobilized prior to its injection into the cytoplasm of the egg by knicking its tail. Instead of using the micropipette to perform this procedure, the laser beam is focused on the sperm tail thus immobilizing it in a few milliseconds without damage to the rest of the sperm. Sperm tail immobilization by the laser obviates the need to suspend the sperm in PVP.

The diode laser is also used to make the opening in the zona through which a single cell (blastomere) is aspirated out for genetic analysis.

Who Benefits?

When a hard or thick egg shell (zonaPellucida) is perceived.

Recommended usage on women of older ages (> 35 years of age).

Women with frozen embryos.

Women who have had previous IVF failures.

Women detected with high FSH (Follicle Stimulating Hormone) levels.

The Process

In order to further enhance our pregnancy rates, the laser technology is used for assisted embryo hatching. The Laser beam is focused, so as to thin out “The ZONA” at a point generally 1/4 of the embryos circumference in order to facilitate embryo hatching. The entire procedure is completed in a few milli seconds. Laser beam is focused on the zonapellucida to thin it down to around 10 microns. This thinning in the zonapellucida helps the embryo to escape.

Embryo Transfer

A maximum of 2-3 embryos may be transferred in each cycle after full discussion with the patient in order to reduce the risk of multiple pregnancies. The procedure takes place in the operating theatre but anaesthesia is not usually necessary. Very rarely four embryos are transferred. A speculum is placed in the vagina and the cervix is exposed and cleaned with sterile water. The embryos are then drawn up in to a catheter, which is then carefully and gently inserted through the cervix (neck of the womb) into the uterus. After transfer, the catheter is carefully checked by the embryologist to make sure that all the embryos have been carefully transferred. Embryo transfer is done under ultrasound guidance.

If you have failed IVF in the past, we can use advanced technology to maximize your chances of getting pregnant. At the Fertility Centre, we would like to see each and every patient go back home with their bundle of joy.

Andrology Services

Male Infertility

Male partners with low sperm counts and/or low sperm motility and/or abnormally shaped sperm and/or have antibodies against their own sperm, are classified as “male factor” patients. Specialists in male infertility are called Andrologists and you may need to consult one prior to commencing IVF treatment.

Causes for Male Infertility

Male infertility is very common. About one in twenty men is sub fertile and a male factor is present in half of all infertile couples.

Most infertile men produce low number of sperms which may also show both poor swimming ability (called motility) and are abnormally shaped. In such men, only a small number of normally shaped motile sperm are likely to swim up the woman’s fallopian tube into the vicinity of the egg and even then may be unable to fertilise the egg.

Obstruction to the passage of sperm from the back of the testis to the outside can result from blockage or absence of the vas deferens. Common causes include obviously vasectomy, but any history of injury, and other surgery or sexually transmitted diseases may be important.

Men can make antibodies to their sperm following vasectomy or other trauma or infection. These antibodies are a common cause of infertility and prevent sperm swimming or sticking to the egg.

Some men have difficulties in obtaining an erection or in ejaculation due to a wide range of problems such as diabetes, MS, or previous prostate surgery.

Rarely, a deficiency in the brain pituitary hormones may result in low sperm counts.

Solution: Artificial Insemination

Artificial insemination involves the insertion of the male partner’s semen into the female partner’s uterus in order to improve the chances of pregnancy.